Glycyrrhiza, Licorice, Gan Cao 甘草

Liquorice, Dulcis radix (‘Sweet root’)
Gan Cao (“Sweet root”, TCM)
Yastimadhu (Ayurveda)
Athimathuram (Siddha)
Shing Mnar   ཤིང་མངར  (Tibetan)
Asl us Soos (Unani)
Picture
Picture Ortus Sanitatis, Meydenbach, 1491

Picture New Kreuterbuch, Matthiolus, 1563

Picture G. glabra
Atlas der officinellen pflanzen
(2), Felix, 1899

Picture G. echinata
Darstellung und Beschreibung sammtliche
, Berg, 1858


Picture Three varieties of Licorice root available on the Chinese Market. The bottom is the highest quality being wild Licorice from XinJiang. Picture Honey Fried Licorice (Zhi Gan Cao) as used in Chinese Medicine.

Picture



Licorice juice dried
into round discs with
a sealed image out of
Gerard’s Herbal.


Picture Picture
RUSSIAN LICORICE ROOT
1, Unpeeled root with gray and reddish-brown patches of cork. 2. Peeled root with small fissures. 3. Small peeled root. 4. Wood. 5. Thick cortex.
SPANISH LICORICE ROOT
1. Rhizome showing wrinkled surface. 2. Small bud. 3, Longitudinal section showing central pith and cortex. 4. Cross-section of rhizome.
Squibb’s Atlas of the Official Drugs, Mansfield, 1919

Botanical name:


Glycyrrhiza spp.
There are around 20 species of Glycyrrhiza, a number of species have been used as a source of ‘Licorice’:
  1. G. glabra; regular ‘Licorice’, native to Eurasia
  2. G. echinata (syn. G. inermis, G. macedonica), German, Roman, Hungarian, East European or Chinese Licorice.
  3. G. glabra var. glanulifera, Russian Licorice
TCM uses G. uralensis, G. inflata (‘Chinese Licorice’). G. yunnanensis (‘Yunnan Licorice’) as well as G. glabra and others.
There is sufficient crossover of the above-named species to say they are synonymous in use, although some will be stronger than others.

Parts used:


Root; dehydrated Juice

Temperature & Taste:


Warm, moist. Sweet
“The root of Licorice is moderate in temperament with an inclination towards hotness and moistness”. (Avicenna)

Classifications:


2I. ANTISPASMODIC.    2L. EMOLLIENT.    2S. STRENGTHENING.   
3B. FEBRIFUGE & ANTIPYRETIC.    3E. DIURETIC.    3H. LACTAGOGUE.    3L. ANTI-TUSSIVE.    3O. EMETICS
4d. PECTORAL.    4e. STOMACHIC.    4g. HEPATIC.    4h. NEPHRITIC.    4j. NERVINE

Uses:


1. Strengthens the Lungs, Clears Phlegm, Stops Cough: (TCM, West, Ayurveda)
-dry Cough, Wheezing, Asthma, shortness of Breath, Bronchitis, Pleurisy; especially good for chronic Bronchitis
-Hemoptysis, Pulmonary Tuberculosis
-supportive in Lung Cancer, Emphysema
-Good for these conditions from Lung heat, dryness, or Lung deficiency, with or without Phlegm


2. Clears Heat and Poison, Benefits the Throat: (TCM, West)
-hoarseness, itching, soreness or irritation of the Throat
-a piece of root or the juice can be sucked


3. Clears Heat and Poison: (TCM, West)
-‘It takes away inflammations’. (Gerard)
-Sores, Boils, Eczema, Dermatitis, Impetigo, and other skin inflammations

wide variety of skin conditions, acting similarly to hydro-cortisone.
-‘useful in chronic Fever’ (Avicenna)
-useful in various Allergies and Viral diseases.
-Scorpion Stings. (Ayurveda, Unani)
-Food Poisoning
-Fetal Toxicosis (Li Shi Zhen)
-‘
Neutralizes the toxins of a hundred drugs … It harmonizes 72 stones and 1,200 herbs’ (Ming Yi Bie Lu)

4. Clears Heat, Quenches Thirst: (TCM, West)
-quenches Thirst for which it has been used since
Dioscorides
-Thirst from various causes
-“
Licorice quenches the Thirst due to its moist nature”. (Avicenna)

5. Tonifies the Spleen and Qi, Benefits Digestion: (TCM, West)
-weakness, fatigue and poor appetite associated with digestive weakness
-Peptic ulcers, gastritis, hyperacidity with pain; accepted for Gastro-duodenal Ulcers by Commission E.
-Colitis and Diverticulitis etc.
Rasayana: tonic to the 7 Tissues; gives Strength, promote Complexion and Hair Growth, benefits the Voice and Eyes, and increases Semen

-also as an adjunct in Anemia
-“

6. Strengthen the Kidneys: (TCM, West, Ayurveda)
-Aphrodisiac; increases Semen, good for Sexual Debility (West, Ayurveda)
-Diabetes

-helps promote Ovulation
-useful to help prepare for Birth
Adreno-corticol insufficiency
-adjunct in chronic Joint diseases
-Kidney and Bladder Ulcers
-‘stabilizes tendons and bones’ (Shen Nong Ben Cao)

7. Calms and Benefits the Liver (TCM, West):
-muscular spasm, pain and tension
-Liver congestion, Biliousness
Hepatitis, Cirrhosis, Jaundice and other chronic Liver disorders
-Hypertension
Hildegard said it ‘extinguishes Anger and Aggression’ and ‘Calms the Mind and Senses’
-‘
facilitates the normal flow of the blood and Qi’. (Ming Yi Bie Lu)

8. Benefits the Heart Qi (TCM, Ayurveda, West):
weakness or deficiency of the energy or blood with irregular pulse and/or palpitations
-breathlessness associated with Heart weakness
-‘
Long-term intake of the drug enables the person to feel happy and enjoy a long life‘ (Shen Nong Ben Cao)
-‘pacifies the Mind and Soul’. (Da Ming)
-‘palpitations, restlessness, amnesia’. (Da Ming)

9. Promotes Milk:
-adjunct to promote Lactation (Ayurveda)

10. Emetic:
-in Panchakarma of Ayurveda (cleansing therapies), large doses of Licorice decoction are used as an emetic.

11. Harmonises, Moderates and Guides Medicines, Moderates Poison:
-softens bitter or strong-tasting medicines, making them more acceptable to the Stomach
-‘It is likewise joined with other Medicines, as well to moderate their Force, as to render them more agreeable. (A Treatise on Foreign Drugs, Geoffroy and Thicknesse, 1749)
-in TCM to moderate the effect of violent, harsh or toxic herbs (some cathartics being the main exceptions)
-specifically moderates Aconite and Arisaema species; also Datura, Coffee, Tobacco, Cocaine and Nux Vomica.
Salmon said ‘it makes purging Physick work without pain’.
-balances and guides a formula; ensures medicines are led to their relevant areas, organs, and channels of the body.
-good to use with excessively hot or cold medicines to moderate them.
-added to complex formulas, where hot and cold medicines, or medicines of a different nature are combined together.


12. Externally:
-root is chewed to cleanse the mouth and teeth, and resist decay.
-dehydrated juice is dissolved under the tongue to quench thirst.
-root is sucked to alleviate the cravings for Tobacco.
-topically for Eczema, Psoriasis and Herpes
-decoction was used as a wash for Erysipelas (Ayurveda)
-wash or eye drops is used for Conjunctivitis, Blepharitis and other inflammations of the eyes
-in hair oils to promote hair growth and for greying hair



Dose:


1. The unprepared medicine is best for clearing Heat and Toxin.
2. The stir-fried root is warmer and more tonifying.
3. The Honey/Sugar-prepared root nourishes and strengthens.
4. The dried juice is best for the throat, coughs and to quench thirst.
Powder: 500mg–3 grams (typically 1–2 grams);
Decoction: 2–9 grams (typically 3–6 grams);
Fluid Extract (1:1): 2–4mls.
The dried juice can be taken in doses of 250mg–1 gram, or can be sucked as needed.

Preparation:


1. Honey-prepared Licorice: (Zhi Gan Cao)
  Licorice root can be stir-fried in Honey or Sugar syrup. This makes the root more tonifying as well as helping to soothe the Lungs and stop Cough.
2. Stir-fried Licorice: (Chao Gan Cao)
  The root is stir-fried until yellowish-red on the inside. This is more warming, and better for Cold Phlegm and Damp conditions and considered better for tonifying. (Li Shi Zhen)
3. Butter/Ghee-prepared Licorice: (Su Gan Cao)
  An old Chinese preparation involved stir-frying Licorice root with about one-third of its weight of butter until the butter is consumed. This is more nourishing and tonifying.
  Based on the Ayurvedic use of butter in such situations, it would be better to use Ghee (clarified butter).
4. Bile-prepared Licorice: (Dan Gan Cao)
  Licorice root has been soaked in Bile to relieve Heat and stop Cough, especially in Children. It is soaked in bile, stir-fried until dry, then powdered. This is rarely used today. (TCM)

Substitute:


1. Mastic and Licorice share similar functions of strengthening the Spleen, increasing Qi, and harmonising medicines.
2. Licorice root or Licorice root extract can generally replace Licorice juice.

Comment:


Two other medicines derived from Licorice are in common use:
1. Licorice juice: Licorice juice is the black, shiny, brittle, sweet dehydrated juice of Licorice. It is sweet, slightly Bitter, and has the general function of Licorice root. However, it excels in the treatment of Lung conditions for which it was most used.
2. Stir-Fried Licorice (Zhi Gan Cao): this is Licorice root that has been stir-fried with Honey (traditionally) or Sugar syrup (more common today) until candied. It is sweet and sticky, and is used in decoctions in TCM. It is most used in Tonic formulas as it makes Licorice stronger to Tonify.

Correction:
1. Tragacanth (or Cochlospermum religiosum) for Kidney diseases (Unani)
2. Rose (Spleen diseases) (Unani)

3. In Ayurveda the precautions for Licorice (mainly edema, hypertension and osteoporosis) are nullified if it is decocted in Milk.

Main Combinations:


Almond & Licorice
Anise & Licorice
Cinnamon & Licorice
Coltsfoot & Licorice
Elecampane & Licorice
Licorice & Comfrey
Peony & Licorice
Raisin & Licorice
Rose, Sandalwood & Licorice

Lungs
1. Cough, Bronchitis, Lung diseases:
i. Syrup of Licorice alone is effective (Herbarium Horstianum, 1630)
ii. Wind-Cold or Spasmodic in Children, decoct Licorice with Aniseed
iii. Licorice, Poippy seed, Mastic
iv. Licorice with Barley, Raisin
v. Licorice with Maidenhair and Figs
vi. Licorice (2 oz.) with Hyssop (1 oz.) and Maidenhair (0.5 oz.); for Cough, Bronchitis, Lung ulcers, Pleurisy
vii. Licorice with Mallow seed, Pumpkin seed, Gum Arabic, Tragacanth, Saffron (as in Pills of Surfa of Unani)
viii. Cough, Hoarseness, loss of Voice, Asthma, spitting of Blood, Licorice with Tragacanth, Sweet Almonds (as in Black Troches)
viiii. Lung congestion, Licorice Basil, Long Pepper, Belleric Myrobalan, Chebulic Myrobalan, Tylophora, Sida (Ayurveda)
x. Cough with Chest Pain, Licorice juice, Dates stones (3 drams each), Starch, Saffron (1 dram each), Tragacanth (2½ drams). Beat and form pills the side of Chickpeas. Take one pill and hold under the tongue. (Syrian “Book of Medicine“, trans. by Wallis Budge, 1913)
2. Acute Sore Throat:
i. decoct Licorice with Burdock seed (TCM)
ii. decoct Licorice with Platycodon Jie Geng and Donkey Hide Gelatin E Jiao. (Ben Cao Gang Mu)
3. Influenza with Cough and Lung congestion, Licorice with fresh Ginger and Ephedra
4. Asthma, Chronic Cough:
i. Licorice, Coltsfoot, Bitter Almond, Maidenhair
ii. Licorice, Elecampane, Raisins, Coltsfoot
iii. Licorice taken with Lohoch of Pine nuts (Herbarium Horstianum, 1630)
5. To strengthen the Lungs:
i. Licorice, Elecampane, Mastic, Cinnamon, Saffron
ii. Licorice, Elecampane, Raisins, Sweet Almonds, Coltsfoot as a decoction
iii. Licorice with Sweet Almond, Pine nut, Hyssop, Maidenhair, Orris, Elecampane with Honey as an Electuary.
6. Consumption, Phthisis:
i. Licorice with Elecampane, and Flowers of Sulphur
ii. Licorice with Comfrey root and Asparagus root
iii. Licorice, Tragacanth, Poppy seed
iv. Licorice, Veronica, Barley, Aniseed (Herbarium Horstianum, 1630)
v. Licorice taken with Sound and Experienced Electuary (Lohoch Sanum et Expertum)
(Herbarium Horstianum, 1630)

Digestion, Tonic
7. Licorice is combined with Rose in a number of formulas to strengthen and harmonise the Stomach and Spleen.
8. Stomach Pain: ‘let him take Licorice root in water without food and he will recover’ (Assyrian text)
9. Gastro-duodenal Ulcers and Inflammation:
i. Licorice, Rose, Sandalwood, Mastic
ii. Licorice with Fennel, Asparagus root, Emblic Myrobalan, Picrorhiza (Ayurveda)
10. Belching, Bloating, Fullness after eating:
i. Licorice with Fennel seed, Aniseed, Coriander, Cinnamon, Galangal
ii. Licorice with Caraway, Aniseed, Coriander, Ginger, Clove
11. Strengthen the Spleen and increase Qi:
i. Licorice with Atractylodes Bai Zhu, Codonopsis Dang Shen, Poria Fu Ling (as in Si Jun Zi Tang)
ii. Licorice with Elecampane, Fennel seed, Ginger
iii. Licorice with Withania, Sida cordifolia, Asparagus root (Ayurveda)
12. To promote Longevity, Virility and Intellect, and cure diseases of aging, combine Licorice with Tabasheer, Rock Salt, Long Pepper, Triphala (Ayurveda)
13. To promote Intelligence and as a Aphrodisiac, take 10 grams of Licorice powder with Milk and Honey (Ayurveda)
14. Anemia:
i. take Licorice with Honey (Ayurveda)
ii. Licorice, Date, Almond, Cinnamon
iii. Licorice with Dang Gui, Astragalus (TCM)

Spasms:
15. Muscular pain, spasm and tension, cramps:
i. Licorice with Peony (this is a stand alone formula for this purpose in TCM)
ii. Licorice, Valerian, Peony

Heart:
16. Heart tonic:
i. Licorice with Cinnamon (TCM)
ii. Licorice, Cinnamon, Mastic, Saffron
iii. Licorice with Picrorhiza (Ayurveda, Charaka)
17. Palpitations:
i. with slow, uneven pulse, decoct Licorice alone and drink daily (Ben Cao Gang Mu)
ii. from Cold and Yang deficiency Licorice with Cinnamon (TCM)

Poisoning:
18. Poisoning from drugs: soak Licorice root in Sesame oil, the longer the better. Let the patient chew the drug and swallow the saliva (Ben Cao Gang Mu)
19. Acute Food Poisoning and other acute Poisoning, decoct Licorice 10 grams with Green Mung beans 30 grams for 10 minutes; drink the Soup. (Chinese Kitchen remedy)

Other:
20. Thirst:
i. Liorice root or juice held in the mouth
ii. Licorice decocted in Barley water (Herbarium Horstianum, 1630)
iii. Licorice, Tragacanth, form troches to hold in the mouth

21. Edema:
i. combine Licorice with Sesame seed (Ayurveda)
ii. from Cold and Damp, Licorice, Celery seed, Parsley seed, Fennel seed, Ginger, Cinnamon
22. Emollient for Stones, Licorice decocted in Barley water with Raisins (Herbarium Horstianum, 1630)
23. Liver diseases:
i. Licorice with Madder, Long Pepper, Tinospora (Ayurveda)
ii. Licorice with Scutellaria Huang Qin, Paeonia Bai Shao (TCM)
iii. Licorice with Agrimony, Gentian, Fennel seed
24. Bleeding, vomiting Blood from Heat, Licorice with White Sandalwood, with Milk (Ayurveda, Chakradatta)
25. To prepare for Birth:
i. take equal parts of Cinnamon and Licorice daily for 2 weeks before the due date. (Culpeper)
ii. Licorice and Raspberry leaf tea, taken for 2 weeks before the due date
26. To promote Milk in nursing mothers:
i. take Licorice powder with Milk (Ayurveda)
ii. Licorice, Fennel, Aniseed

External:
27. Baldness or Alopecia, make a paste of Licorice powder with Saffron in Milk. Apply topically every night. (Ayurveda)
28. Licorice is used with Eclipta in hair oils to promote Hair growth, and for premature greying Hair. (Ayurveda)
29. Wounds, Bruises, Burns, Licorice powder is mixed with Ghee and applied
30, As an eyewash, Licorice with Triphala

Major Formulas


Infusion of Dr. Gill
Common Decoction
Decoction for Cough (Gabelhover)
Syrup Against Consumption
Syrup of Licorice (Mesue)
Syrup of Maidenhair
Pectoral Powder
Powder for Cold Cough
Powder of Licorice (Unani)
Black Troches for Cough (Trochisci Bechici) (Mesue)
Decoction to Strengthen the Lungs
Pills for Hot Cough (Zacharia)

Barberry 8 Powder (Skyer sun brgyad pa) (Tibetan)
Blood Medicine 7 (Khrag sman bdun pa) (Tibetan)
Clove 6 (Li shi drug pa) (Tibetan)
Licorice 6 (Shing mngar drug pa) (Tibetan)
Pomegranate 9 Decoction (Se bru dgu thang) (Tibetan)
Raisin 7 (Tibetan)
Sandalwood 8 (Tsan dan brgyad pa) (Tibetan)
Sea Buckthorn 5 (Star bu 5) (Tibetan)
Settle the Lungs 8 (Glo man brgyad pa) (Tibetan)
Tabasheer 9 (Tibetan)
Zhi Gan Cao Tang
Gan Cao Xie Xin Tang
Shao Yao Gan Cao Tang
Ba Zhen Tang
Bai He Gu Jin Tang
Bu Gan Tang
Bu Zhong Yi Qi Tang
Chai Hu Shu Gan San
Da Qing Long Tang
Er Chen Tang
Gou Teng San
Gui Zhi Tang
Gui Zhi Fu Zi Tang
Huang Lian Wen Dan Tang
Li Zhong Wan
Ling Gan Wu Wei Jiang Xin Tang
Ling Gui Zhu Gan Tang
Liu Jun Zi Tang
Ma Huang Tang
Ma Xing Shi Gan Tang
Ping Wei San
Ren Shen Yang Rong Tang
Shen Ling Bai Zhu San
Shi Quan Da Bu Tang
Si Jun Zi Tang
Si Ni San
Tai Shan Pan Shi San
Xiang Sha Liu Jun Zi Tang
Xiao Chai Hu Tang
Xiao Qing Long Tang
You Gui Yin
Zhi Sou San
Zuo Gui Yin

1. White Pectoral Troches:
i. Licorice root powder (1 oz.), Orris root powder (½ oz.), Sugar Candy (1 lb.), Mucilage of Tragacanth (sufficient). Make Troches.
ii. Licorice root powder (3 drams), Orris root powder (6 drams), Starch (½ oz.), White Sugar (8 oz.), mucilage of Tragacanth (sufficient) (Pharmacopoeia Suecica, 1817)
iii. Licorice root powder (1 ½ oz.), Orris root powder, Gum Arabic (½ oz. each), powdered White Sugar (48 oz.), mucilage of Tragacanth (sufficient). (Pharmacopoeia Generalis, 1783)

2. Black Pectoral Troches:
i. Licorice juice, Gum Arabic (equal parts). dissolve each separately in water, mix the liquors and evaporate to the proper consistency.
ii. Extract of Licorice, Gum Arabic (1 part each), Sugar (2 parts), Water (sufficient). evaporate the strained solution. (Edinborough)

3. Compound Licorice Troches:
i. Orris root, Licorice root, Aniseed, Fennel seed (1 oz. each), Licorice juice dissolved in Hyssop water (4 oz.), White Sugar (16 oz.), mucilage of Tragacanth (sufficient). (Dispensatorium Pharmaceuticum, 1777)

4. White Licorice Sticks:
i. Gum Arabic, Diambra, Diatragacanth (½ oz. each), Licorice powder, Starch (1 lb. each), White Sugar (2 lbs.), mucilage of Tragacanth (sufficient). Form sticks. (Pharmacopoeia Generalis, 1783)

5. Yellow Pectoral Troches:
i. Licorice root, Florentine Orris (1 oz. each), Saffron (2 scruples), White Sugar (1 lb.), mucilage of Tragacanth (sufficient). Make Troches, (Pharmacopoeia Generalis, 1783)
ii. Licorice (8 oz.), Diatragacanth, Florentine Orris (1 ½ oz. each), Saffron (8 scruples), Sugar (6 pounds), mucilage of Tragacanth (sufficient).

Cautions:


1. Not used in Phlegm conditions (without correction)
2. Full doses are not generally used on an extended basis; smaller doses as in powders is better for long-term use.
3. Edema, osteoporosis, and hypertension may be caused or aggravated by long-term use or large doses.
4. Incompatible with Euphoriba and Daphne species. (TCM)

Drug Interactions:


1. Can increase potassium loss when used with potassium-depleting drugs (diuretics)
2. Can cause increased sensitivity to cardiac medication (due to potassium loss)
3. Increases clearance time of corticosteroids, so care on people using steroids, especially prednisolone.
4. Can potentially counteract contraceptive pill.
5. Counteracts negative effects of NSAID’s on gastric mucosa (ie. inhibits irritations and ulceration)

Toxicity


No adverse effects were noted in single doses of up to 5000mg/kg of a standardized extract given to rats. Subchronic toxicity studies of 90 days revealed no adverse reactions at up to 1000mg/kg per day. (see here)
Toxicological Effects of Glycyrrhiza glabra (Licorice): A Review.

Main Preparations used:


Licorice Juice, Syrup, Black Troches

1. Licorice Juice:
i. prepared by exhausting the root with several decoctions, combining the decoctions and inspissating until thick.
ii. Licorice powder 4 oz., Tragacanth dissolved in Hyssop water 6 oz. Infuse 24 hours, strain and dry in the sun until it grows hard and roll into cakes. (Natura exenterata, Philiatros, 1655)

2. Purified Licorice Juice:
i. dissolve in hot water, strain, evaporate to a firm mass and roll into cylinders on an oiled marble slab. Dry with gentle heat, then cut into sticks. (Pharmacopee Usuelle, Louvain, 1821)

3. Licorice juice with Gum:
i. Gum Arabic, Licorice juice (equal parts). Dissolve each separately in water, strain, mix and evaporate to a firm mass. (Pharmacopee Usuelle, Louvain, 1821)

4. Anisated Licorice juice:
i. Depurated Licorice juice while still warm (48 oz.), Anise oil (3 drams); mix, and roll into thin sticks and dry. (Pharmacopee Usuelle, Louvain, 1821)

5. Honey of Licorice:
i. Licorice root (8 oz.), Boiling Water (3 pounds); macerate 1 hour, express, evaporate to 14 oz., and add Honey (5 lbs.). Boil to a syrup. (Pharmacopoeia regni Poloniae, 1817)

6. Syrup of Licorice:
i. Licorice root (1 part), Water (3 parts), infuse an hour in a water-bath, strain, press, and dissolve in the infusion when cold White Sugar (2 parts)

7. Licorice Extract:
i. Licorice root (1 pound), Boiling Water (1 gallon). Macerate 24 hours, boil to 4 pints. Strain while hot and evaporate to an extract. (London)
ii. some decocted the residue again with fresh water, combined the decoctions, then evaporated.

8. Licorice Paste:
i. Grated Licorice (4 oz.), Boiling Water (8 oz.). Macerate 12 hours, strain, then dissolve in Gum Arabic (2 ½ lbs.), White Sugar (1 ½ pounds). Strain, and evaporate slowly until a drop thrown onto a cold glass plate gelatinises. Then pour the mass into oiled metallic molds, dry, then cut. (Pharmacopoeia Oldenburgica, 1801)
ii. Licorice (4 oz.), Water (32 oz.), boil to 24 oz., and dissolve in the strained liquor Gum Arabic (2 lbs.), White Sugar (1 ½ lbs.). Boil to a proper consistency. (Dispensatorium Pharmaceuticum, 1777)

9. Licorice Lozenges:
i. Licorice extract (1 part), Sugar, liquified over the fire (16 parts); pulverise the extract and mix into the sugar and roll the lozenges in starch. (Pharmacopee Usuelle, Louvain, 1821)
Picture Packages of imported Licorice root from Persia.
A Manual of Organic Materia Medica and Pharmacognosy, Sayre, 1907

  • Extra Info
  • History
  • Research
  • Licorice Juice
From Pharmacographia Indica, Dymock, 1890
Liquorice grows wild in Arabia, Persia, Turkistan and Afghanistan, and has been introduced in to the Punjab and Sind. Kinneir observed it growing abundantly near Basra, and Aitchison found it growing abundantly all over the Badghis and throughout the Harirud and Khorasan districts. In Persia glass-bottle-makers use the wood for melting their materials, as they say it gives a greater heat than any other kind of fuel. The root, in Sanskrit called Yashtimadhu and Madhuka, must have been known to the Hindus from a very early date, as it is mentioned by Susruta. Hindu works describe it as demulcent, cooling and useful in cough, hoarseness, &c. It is also recommended as a flavouring agent, and enters into the composition of many external cooling applications.

Abu Hanifeh describes Sus as a well-known plant, the expressed juice of which is an ingredient in medicine. He says the roots are sweet and the branches bitter. El Mutarrizi in the Mughrib states that the leaves are put into the beverage called nabid to make it strong. The modern Arabs call the root Irk-es-sus, and make a
strong infusion of it which they drink. The dried juice is called Rab-es-sus; it is made by the Arabs, Turkomans, and Persians at Yezd. In Persia the liquorice plant is called Mehak and Mazhu.

The author of the Makhzan-el-Adwiya gives a lengthy description of the plant, and directs the root to be decorticated before it is used. He says that the Egyptian is the best, next that of Irak, and then Syrian. The root is considered hot, dry and suppurative, demulcent and lenitive, relieving thirst and cough, and removing unhealthy humours, also diuretic and emmenagogue, useful in asthma and irritable conditions of the bronchial passages. Ibn Sina recommends the decoction in cold colic; it is also dropped into the eyes to strengthen the sight. A poultice made of the leaves is said to be a cure for scald head, and stinking of the feet or armpits. Muhammad bin Ahmad and Yohanna bin Serapion recommend the seeds as being the most active part of the plant’.

From Pharmacographia, Fluckiger & Hanbury, 1879
1. Licorice root
‘Theophrastus a in commenting on the taste of different roots (3rd cent. B.C.) instances the sweet Scythian root which grows in the neighbourhood of the lake Maeotis (Sea of Azov), and is good for asthma, dry cough and all pectoral diseases,— an allusion unquestionably to liquorice. Dioscorides, who calls the plant [?] notices its glutinous leaves and purplish flowers, but as he describes the pods to be in balls resembling those of the plane, and the roots to be sub-austere as well as sweet, it is possible he had in view Glycyrrhiza echinata L. as well as G. glabra.

Roman writers, as Celsus and Scribonius Largus, mention liquorice as Radix dulcis. Pliny, who describes it as a native of Cilicia and Pontus, makes no allusion to it growing in Italy.

The cultivation of liquorice in Europe does not date from a very remote period, as we conclude from the absence of the name in early mediaeval lists of plants. It is, for instance, not enumerated among the plants which Charlemagne ordered (a.d. 812) to be introduced from Italy into Central Europe; nor among the herbs of the convent gardens as described by Walafridus Strabus, abbot of Reichenau, lake of Constance, in the 9th century; nor yet in the copious

list of herbs contained in the vocabulary of Alfric, archbishop of Canterbury in the 10th century.

On the other hand, liquorice is described as being cultivated in Italy by Pierode Crescenzi] of Bologna, who lived in the 13th century. The cultivation of the plant in the north of England existed at the close of the 16th century, but how much earlier we have not been able to trace.


As a medicine the drug was well known in Germany in the 11th century, and an extensive cultivation of the plant was carried on near Bamberg, Bavaria, in the 16th century, so that in many of the numerous pharmaceutical tariffs of those times in Germany not only Glycyrrhizoe succus creticus, seu candiacus, seu venetus is quoted, but also expressly that of Bamberg.

The word Liquiritia, whence is derived the English name Liquorice (Lycorya in the 13th century), is a corruption of Glycyrrhiza, as shown in the transitional mediaeval form Gliquiricia. The Italian Regolizia, the German Lacrisse or Lakriz, the Welsh Lacris, and the French Reglisse (anciently Requelice or Recolice) have the same origin’.

2. Licorice Juice

‘Inspissated liquorice juice was known in the time of Dioscorides, and may be traced in the writings of Oribasius and Marcellus Empiricus in the latter half of the 4th century, and in those of Paulus Aegineta in the 7th. It appears to have been in common use in Europe during the middle ages. In A.D. 1264, “Liquorice” is charged in the Wardrobe Accounts of Henry III.; and as the article cost 3d. per lb.,or the same price as grains of paradise and one-third that of cinnamon, we are warranted in supposing the extract and not the mere root is intended. Again, in the Patent of Pontage granted by Edward L, A.D. 1305, to aid in repairing the London Bridge, permission is given to lay toll on various foreign commodities including Liquorice. A political song written in 1436 makes mention of Liquorice as a production of Spain, but the plant is not named as an object of cultivation by Herrera, the author of a work on Spanish
agriculture in 1513. Saladiuus, who wrote about the middle of the 15th century, names it among the wares kept by the Italian apothecaries ; and it is enumerated in a list of drugs of the city of Frankfort written about the year 1450.

Dorsten, in the first half of the 16th century, mentions the

liquorice plant as abundant in many parts of Italy, and describes the method of making the Succus by crushing and boiling the fresh root. Mattioli states that the juice made into pastilli was brought every year from Apulia, and especially from the neighbourhood of Monte Gargano. Extract of liquorice was made at Bamberg in Germany, where the plant is still largely cultivated, as early as 1560.’ (Pharmacographia, Fluckiger & Hanbury, 1879)
GENERAL / REVIEW
Liquorice (Glycyrrhiza glabra): the journey of the sweet root from Mesopotamia to England.
Glycyrrhiza glabra (Licorice): A Comprehensive Review on Its Phytochemistry, Biological Activities, Clinical Evidence and Toxicology.
Glycyrrhiza Genus: Enlightening Phytochemical Components for Pharmacological and Health-Promoting Abilities.
Isolation, structural characterization, biological activity, and application of Glycyrrhiza polysaccharides: Systematic review.
Traditional Uses, Bioactive Chemical Constituents, and Pharmacological and Toxicological Activities of Glycyrrhiza glabra L. (Fabaceae).
A Comprehensive Review for Phytochemical, Pharmacological, and Biosynthesis Studies on Glycyrrhiza spp.
Isolations, characterizations and bioactivities of polysaccharides from the seeds of three species Glycyrrhiza.
Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review.
The genetic and chemical diversity in three original plants of licorice, Glycyrriza uralensis Fisch., Glycyrrhiza inflata Bat. and Glycyrrhiza glabra L.
Review – Glycyrrhiza glabra L. (Liquorice).
Pharmacological Effects of Glycyrrhiza spp. and Its Bioactive Constituents: Update and Review.

ANTIBACTERIAL
Isolation and antimicrobial activities of actinobacteria closely associated with liquorice plants Glycyrrhiza glabra L. and Glycyrrhiza inflate BAT. in Xinjiang, China.
Comparative analysis of antibacterial properties and chemical composition of Glycyrrhiza glabra L. from Astrakhan region (Russia) and Calabria region (Italy).

ANTI-VIRAL
Revisiting liquorice (Glycyrrhiza glabra L.) as anti-inflammatory, antivirals and immunomodulators: Potential pharmacological applications with mechanistic insight.
Uralsaponins M-Y, antiviral triterpenoid saponins from the roots of Glycyrrhiza uralensis.
COVID
Licorice (Glycyrrhiza glabra) Extracts-Suitable Pharmacological Interventions for COVID-19? A Review.
Chemical composition and pharmacological mechanism of ephedra-glycyrrhiza drug pair against coronavirus disease 2019 (COVID-19).
Coronavirus-19: Possible Therapeutic Implications of Spironolactone and Dry Extract of Glycyrrhiza glabra L. (Licorice).
Molecular docking and ADMET study of bioactive compounds of Glycyrrhiza glabra against main protease of SARS-CoV2.
COXSACKIE
Glycyrrhizic acid as the antiviral component of Glycyrrhiza uralensis Fisch. against coxsackievirus A16 and enterovirus 71 of hand foot and mouth disease.
ENTEROVIRUS
Glycyrrhizic acid as the antiviral component of Glycyrrhiza uralensis Fisch. against coxsackievirus A16 and enterovirus 71 of hand foot and mouth disease.
HEPATITIS
Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species.
HERPES
The Effect of Aqueous Extract of Glycyrrhiza glabra on Herpes Simplex Virus 1.
INFLUENZA:
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus.
NEWCASTLE
In vivo antiviral potential of Glycyrrhiza glabra extract against Newcastle disease virus.
Comparative study to evaluate the anti-viral efficacy of Glycyrrhiza glabra extract and ribavirin against the Newcastle disease virus.
ROTAVIRUS
Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea.

ANTI-FUNGAL
Fungicidal Activity and Mechanism of Action of Glabridin from Glycyrrhiza glabra L.

ANTI-MALARIAL
Antiplasmodial Property of Glycyrrhiza glabra Traditionally Used for Malaria in Iran: Promising Activity with High Selectivity Index for Malaria.
In silico and in vivo anti-malarial studies of 18β glycyrrhetinic acid from Glycyrrhiza glabra.

ANTI-INFLAMMATORY
The Anti-Inflammatory Properties of Licorice (Glycyrrhiza glabra)-Derived Compounds in Intestinal Disorders.
Revisiting liquorice (Glycyrrhiza glabra L.) as anti-inflammatory, antivirals and immunomodulators: Potential pharmacological applications with mechanistic insight.
NLRP3 Inflammasome Pharmacological Inhibitors in Glycyrrhiza for NLRP3-Driven Diseases Treatment: Extinguishing the Fire of Inflammation.
Effects of a pinitol-rich Glycyrrhiza glabra L. leaf extract on insulin and inflammatory signaling pathways in palmitate-induced hypertrophic adipocytes.
Antioxidant and Anti-Inflammatory Activities of Flavanones from Glycyrrhiza glabra L. (licorice) Leaf Phytocomplexes: Identification of Licoflavanone as a Modulator of NF-kB/MAPK Pathway.
Constituents Isolated from the Leaves of Glycyrrhiza uralansis and Their Anti-Inflammatory Activities on LPS-Induced RAW264.7 Cells.
Evidence for Anti-Inflammatory Activity of Isoliquiritigenin, 18β Glycyrrhetinic Acid, Ursolic Acid, and the Traditional Chinese Medicine Plants Glycyrrhiza glabra and Eriobotrya japonica, at the Molecular Level.
Chemical Profile and Anti-inflammatory Activity of Total Flavonoids from Glycyrrhiza Uralensis Fisch.

ANTI-ALLERGIC
Effect of hot water extract of a glycyrrhizin-deficient strain of Glycyrrhiza uralensis on contact hypersensitivity in mice.
Evaluation of the safety and efficacy of Glycyrrhiza uralensis root extracts produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems III: anti-allergic effects of hot water extracts on IgE-mediated immediate hypersensitivity in mice.

ANTIOXIDANT
Antioxidant and Anti-Melanogenic Activities of Heat-Treated Licorice (Wongam, Glycyrrhiza glabra × G. uralensis) Extract.
The antioxidant effectiveness of liquorice (Glycyrrhiza glabra L.) extract administered as dietary supplementation and/or as a burger additive in rabbit meat.
Antioxidant and Anti-Inflammatory Activities of Flavanones from Glycyrrhiza glabra L. (licorice) Leaf Phytocomplexes: Identification of Licoflavanone as a Modulator of NF-kB/MAPK Pathway.
Eight new triterpenoid saponins with antioxidant activity from the roots of Glycyrrhiza uralensis Fisch.
Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.

IMMUNOMODULATORY / IMMUNOSTIMULANT
Revisiting liquorice (Glycyrrhiza glabra L.) as anti-inflammatory, antivirals and immunomodulators: Potential pharmacological applications with mechanistic insight.
Effect of Licorice (Glycyrrhiza glabra) Extract as an Immunostimulant on Serum and Skin Mucus Immune Parameters, Transcriptomic Responses of Immune-Related Gene, and Disease Resistance Against Yersinia ruckeri in Rainbow Trout (Oncorhynchus mykiss).
The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis.
Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.
The immunomodulatory activities of licorice polysaccharides (Glycyrrhiza uralensis Fisch.) in CT 26 tumor-bearing mice.
Immunomodulatory and anticancer potential of Gan cao (Glycyrrhiza uralensis Fisch.) polysaccharides by CT-26 colon carcinoma cell growth inhibition and cytokine IL-7 upregulation in vitro.

ANTI-TOXIN–ACONITE, NUX VOMICA, CADMIUM ETC
Glycyrrhiza uralensis promote the metabolism of toxic components of Aconitum carmichaeli by CYP3A and alleviate the development of chronic heart failure.
Glycyrrhiza uralensis Fisch. and its active components mitigate Semen Strychni-induced neurotoxicity through regulating high mobility group box 1 (HMGB1) translocation.
The genus Glycyrrhiza (Fabaceae family) and its active constituents as protective agents against natural or chemical toxicities.
Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats.
In vitro studies on protective effect of Glycyrrhiza glabra root extracts against cadmium-induced genetic and oxidative damage in human lymphocytes.

ANTITHROMBOTIC
Antithrombotic phenolic compounds from Glycyrrhiza uralensis.

CARDIOPROTECTIVE
Cardioprotective effects of Glycyrrhiza uralensis extract against doxorubicin-induced toxicity.

MYOCARDIAL ISCHEMIA
Glycyrrhiza glabra protects from myocardial ischemia-reperfusion injury by improving hemodynamic, biochemical, histopathological and ventricular function.

RESPIRATORY TRACT INFECTIONS
Pharmacological Efficacy and Safety of Glycyrrhiza glabra in the treatment of respiratory tract infections.
Effect of aquo-alchoholic extract of Glycyrrhiza glabra against Pseudomonas aeruginosa in Mice Lung Infection Model.

ALLERGIC RHINITIS
Nasal irrigation with Glycyrrhiza glabra extract for treatment of allergic rhinitis – A study of in vitro, in vivo and clinical trial.

ACUTE SINUSITIS
Treatment of a Woman With Glycyrrhiza glabra for Acute Sinusitis: A Case Report.

COPD
Compound Glycyrrhiza Oral Solution alleviates oxidative stress and inflammation by regulating SRC/MAPK pathway in chronic obstructive pulmonary disease.

TUBERCULOSIS
Inhalable liposomes of Glycyrrhiza glabra extract for use in tuberculosis: formulation, in vitro characterization, in vivo lung deposition, and in vivo pharmacodynamic studies.
Anti-tubercular agents from Glycyrrhiza glabra.

PULMONARY FIBROSIS
The effects of methanolic extract of Glycyrrhiza glabra on the prevention and treatment of bleomycin-induced pulmonary fibrosis in rat: experimental study.

HEPATOPROTECTIVE
Hepatoprotective effect of total flavonoids from Glycyrrhiza uralensis Fisch in liver injury mice.
Protective effects of Glycyrrhiza glabra supplementation against methotrexate-induced hepato-renal damage in rats: An experimental approach.
Isolation, structural elucidation and in vitro hepatoprotective activity of flavonoids from Glycyrrhiza uralensis.
Hepatoprotective and antioxidant effects of dietary Glycyrrhiza polysaccharide against TCDD-induced hepatic injury and RT-PCR quantification of AHR2, ARNT2, CYP1A mRNA in Jian Carp (Cyprinus carpio var. Jian).
Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease.
Hepatoprotective triterpene saponins from the roots of Glycyrrhiza inflata.
Hepatoprotective Effects of Silybum marianum (Silymarin) and Glycyrrhiza glabra (Glycyrrhizin) in Combination: A Possible Synergy.

HEPATIC FIBROSIS
Structures and In Vitro Antihepatic Fibrosis Activities of Prenylated Dihydrostilbenes and Flavonoids from Glycyrrhiza uralensis Leaves.

HYPNOTIC
Hypnotic effects and GABAergic mechanism of licorice (Glycyrrhiza glabra) ethanol extract and its major flavonoid constituent glabrol.

ANTI-CONVULSANT
Anti-convulsant action and amelioration of oxidative stress by Glycyrrhiza glabra root extract in pentylenetetrazole- induced seizure in albino rats.

NEUROPROTECTIVE
Glycyrrhiza uralensis Fisch. and its active components mitigate Semen Strychni-induced neurotoxicity through regulating high mobility group box 1 (HMGB1) translocation.
Metabolomics analysis highlights Yashtimadhu (Glycyrrhiza glabra L.)-mediated neuroprotection in a rotenone-induced cellular model of Parkinson’s disease by restoring the mTORC1-AMPK1 axis in autophagic regulation.
Data on dose-dependent cytotoxicity of rotenone and neuroprotection conferred by Yashtimadhu (Glycyrrhiza glabra L.) in an in vitro Parkinson’s disease model.
Prevention of MEK-ERK-1/2 hyper-activation underlines the neuroprotective effect of Glycyrrhiza glabra L. (Yashtimadhu) against rotenone-induced cellular and molecular aberrations.
Identification of Molecular Network Associated with Neuroprotective Effects of Yashtimadhu (Glycyrrhiza glabra L.) by Quantitative Proteomics of Rotenone-Induced Parkinson’s Disease Model.
Chinese Herbal Medicine Glycyrrhiza inflataReduces Aβ Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation.

LEARNING & MEMORY
Beneficial effect of aqueous root extract of Glycyrrhiza glabra on learning and memory using different behavioral models: An experimental study.
DEPRESSION
Adjunct Therapy With Glycyrrhiza Glabra Rapidly Improves Outcome in Depression-A Pilot Study to Support 11-Beta-Hydroxysteroid Dehydrogenase Type 2 Inhibition as a New Target.
[Antidepressant activities of flavonoids from Glycyrrhiza uralensis and its neurogenesis protective effect in rats].

PARKINSON’S DISEASE
Metabolomics analysis highlights Yashtimadhu (Glycyrrhiza glabra L.)-mediated neuroprotection in a rotenone-induced cellular model of Parkinson’s disease by restoring the mTORC1-AMPK1 axis in autophagic regulation.
Data on dose-dependent cytotoxicity of rotenone and neuroprotection conferred by Yashtimadhu (Glycyrrhiza glabra L.) in an in vitro Parkinson’s disease model.

ALZHEIMER’S DISEASE
Licochalcone B, a chalcone derivative from Glycyrrhiza inflata, as a multifunctional agent for the treatment of Alzheimer’s disease.

GASTROPROTECTIVE
Gastroprotective and gastric motility benefits of AD-lico/Healthy Gut™ Glycyrrhiza inflata extract.

DYSPEPSIA
An Extract of Glycyrrhiza glabra (GutGard) Alleviates Symptoms of Functional Dyspepsia: A Randomized, Double-Blind, Placebo-Controlled Study.

GASTRIC ULCER
Multipathway Integrated Adjustment Mechanism of Glycyrrhiza Triterpenes Curing Gastric Ulcer in Rats.
Antiulcer properties of Glycyrrhiza glabra L. extract on experimental models of gastric ulcer in mice.
The healing effect of licorice (Glycyrrhiza glabra) on Helicobacter pylori infected peptic ulcers.
In vitro anti-Helicobacter pylori activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action.

IMPROVES GUT HEALTH
Effects of dietary Glycyrrhiza polysaccharide supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets.
Effects of Glycyrrhiza Polysaccharides on Chickens’ Intestinal Health and Homeostasis.
Polysaccharides derived from Astragalus membranaceus and Glycyrrhiza uralensis improve growth performance of broilers by enhancing intestinal health and modulating gut microbiota.
A flavonoid rich standardized extract of Glycyrrhiza glabra protects intestinal epithelial barrier function and regulates the tight-junction proteins expression.
Effect of Flavonoid-Rich Extract of Glycyrrhiza glabra on Gut-Friendly Microorganisms, Commercial Probiotic Preparations, and Digestive Enzymes.

IMPROVES GROWTH PERFORMANCE
Effects of dietary Glycyrrhiza polysaccharide on growth performance, blood parameters and immunity in weaned piglets.
Effects of dietary Glycyrrhiza polysaccharide supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets.

INFLAMMATORY BOWEL DISEASE / ULCERATIVE COLITIS
The Anti-Inflammatory Properties of Licorice (Glycyrrhiza glabra)-Derived Compounds in Intestinal Disorders.
Glycyrrhiza Polysaccharide Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
Effect of Glycyrrhiza uralensis against ulcerative colitis through regulating the signaling pathway of FXR/P-gp.
Total Flavonoids of Glycyrrhiza uralensis Alleviates Irinotecan-Induced Colitis via Modification of Gut Microbiota and Fecal Metabolism.
Augmented reduction in colonic inflammatory markers of dextran sulfate sodium-induced colitis with a combination of 5-aminosalicylic acid and AD-lico™ from Glycyrrhiza inflata.

RENOPROTECTIVE
Glycyrrhiza uralensis root extract ameliorates high glucose-induced renal proximal tubular fibrosis by attenuating tubular epithelial-myofibroblast transdifferentiation by targeting TGF-β1/Smad/Stat3 pathway.
Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice.
Protective effects of Glycyrrhiza glabra supplementation against methotrexate-induced hepato-renal damage in rats: An experimental approach.

DIABETES
Effects of a pinitol-rich Glycyrrhiza glabra L. leaf extract on insulin and inflammatory signaling pathways in palmitate-induced hypertrophic adipocytes.
Glycyrrhiza glabra alcoholic root extract ameliorates hyperglycemia, hyperlipidemia, and glycation-induced free iron-mediated oxidative reactions.
Antihyperglycemic Activity of Hydroalcoholic Extracts of Selective Medicinal Plants Curcuma longa, Lavandula stoechas, Aegle marmelos, and Glycyrrhiza glabra and Their Polyherbal Preparation in Alloxan-Induced Diabetic Mice.

METABOLIC SYNDROME
A Review of Glycyrrhiza glabra (Licorice) Effects on Metabolic Syndrome.

ANTI-OBESITY
Anti-obesity potential of Glycyrrhiza uralensis and licochalcone A through induction of adipocyte browning.
The Effect of Dried Glycyrrhiza Glabra L. Extract on Obesity Management with Regard to PPAR-γ2 (Pro12Ala) Gene Polymorphism in Obese Subjects Following an Energy Restricted Diet.

OSTEOARTHRITIS
Prenylated phenolic compounds from licorice (Glycyrrhiza uralensis) and their anti-inflammatory activity against osteoarthritis.

OSTEOPOROSIS
Protective effect of Glycyrrhiza glabra roots extract on bone mineral density of ovariectomized rats.

RHEUMATOID ARTHRITIS
Liquiritin from Glycyrrhiza uralensis Attenuating Rheumatoid Arthritis via Reducing Inflammation, Suppressing Angiogenesis, and Inhibiting MAPK Signaling Pathway.

ESTROGENIC
Effects of licorice on sex hormones and the reproductive system.
Agonistic and antagonistic estrogens in licorice root (Glycyrrhiza glabra).

UTERINE RELAXANT
Analgesic and uterine relaxant effects of isoliquiritigenin, a flavone from Glycyrrhiza glabra.

DYSMENORRHEA
The effect of Glycyrrhiza glabra L. on Primary Dysmenorrhea compared with Ibuprofen: A Randomized, Triple-Blind Controlled Trial.

MENOPAUSE
Licorice (Glycyrrhiza spp.) and jujube (Ziziphus jujuba Mill.) formula for menopausal symptoms: Classical records, clinical evidence and experimental data.
Development of an Improved Menopausal Symptom-Alleviating Licorice (Glycyrrhiza uralensis) by Biotransformation Using Monascus albidulus
Impact of Glycyrrhiza glabra (licorice) vaginal cream on vaginal signs and symptoms of vaginal atrophy in postmenopausal women: A randomized double blind controlled trial.

POLYCYSTIC OVARIES, PCOS
The synergistic effect of Paeonia spp and Glycyrrhiza glabra on polycystic ovary induced in mice.

ENDOMETRIOSIS
Comparing The Effects of Glycyrrhiza glabra Root Extract, A Cyclooxygenase-2 Inhibitor (Celecoxib) and A Gonadotropin-Releasing Hormone Analog (Diphereline) in A Rat Model of Endometriosis.

ANTI-ANDROGENIC (weak)
Effects of licorice on sex hormones and the reproductive system.

EFFECT ON SPERM
Possible ameliorating effects of Glycyrrhiza Glabra (Licorice) on the sperm parameters in rats under high fat diet.

TESTICULAR PROTECTIVE
The modulatory impacts of Glycyrrhiza glabra extract against methotrexate-induced testicular dysfunction and oxidative stress.

PROSTATITIS
Screening of anti-chronic nonbacterial prostatitis activity of different extractions of the aerial part of Glycyrrhiza uralensis, and network pharmacology research.

CANCER
Mechanisms of action of cytotoxic phenolic compounds from Glycyrrhiza iconica roots.
Inhibition effect of glycyrrhiza polysaccharide (GCP) on tumor growth through regulation of the gut microbiota composition.
Total Flavonoids from Radix Glycyrrhiza Exert Anti-Inflammatory and Antitumorigenic Effects by Inactivating iNOS Signaling Pathways.
Phenolic compounds from Glycyrrhiza pallidiflora Maxim. and their cytotoxic activity.
BRAIN
In-vitro antitumor activity of compounds from Glycyrrhiza glabra against C6 glioma cancer cells: identification of natural lead for further evaluation.
BREAST
Glycyrrhiza glabra extract and quercetin reverses cisplatin resistance in triple-negative MDA-MB-468 breast cancer cells via inhibition of cytochrome P450 1B1 enzyme.
COLORECTAL
Immunomodulatory and anticancer potential of Gan cao (Glycyrrhiza uralensis Fisch.) polysaccharides by CT-26 colon carcinoma cell growth inhibition and cytokine IL-7 upregulation in vitro.
Effect of Root Extracts of Medicinal Herb Glycyrrhiza glabra on HSP90 Gene Expression and Apoptosis in the HT-29 Colon Cancer Cell Line.
LIVER
Licochalcone B Extracted from Glycyrrhiza uralensis Fisch Induces Apoptotic Effects in Human Hepatoma Cell HepG2.
Glycyrrhiza polysaccharide induces apoptosis and inhibits proliferation of human hepatocellular carcinoma cells by blocking PI3K/AKT signal pathway.
MELANOMA
Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells.
NASOPHARYNGEAL
Glycyrrhiza glabra suppresses nasopharyngeal carcinoma cell proliferation through inhibiting the expression of lncRNA, AK027294.
ORAL
A polysaccharide from Glycyrrhiza inflata Licorice inhibits proliferation of human oral cancer cells by inducing apoptosis via mitochondrial pathway.
PROSTATE
Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells.

ANTI-METASTATIC
Licoricidin, an Active Compound in the Hexane/Ethanol Extract of Glycyrrhiza uralensis, Inhibits Lung Metastasis of 4T1 Murine Mammary Carcinoma Cells.

SYNERGISTIC WITH CHEMOTHERAPY
Glycyrrhiza glabra extract and quercetin reverses cisplatin resistance in triple-negative MDA-MB-468 breast cancer cells via inhibition of cytochrome P450 1B1 enzyme.

RADIO- AND CHEMO-THERAPY SIDE EFFECTS
Recent Advances in Glycyrrhiza glabra (Licorice)-Containing Herbs Alleviating Radiotherapy- and Chemotherapy-Induced Adverse Reactions in Cancer Treatment.
Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice.
The efficacy of an ayurvedic preparation of yashtimadhu (Glycyrrhiza glabra) on radiation-induced mucositis in head-and-neck cancer patients: A pilot study.
Glycyrrhiza glabra (Licorice) root extract attenuates doxorubicin-induced cardiotoxicity via alleviating oxidative stress and stabilising the cardiac health in H9c2 cardiomyocytes.
Preventive Effect of Glycyrrhiza Glabra Extract on Oral Mucositis in Patients Under Head and Neck Radiotherapy: A Randomized Clinical Trial.
Combination of Nigella sativa with Glycyrrhiza glabra and Zingiber officinale augments their protective effects on doxorubicin-induced toxicity in h9c2 cells.
Protective effect of Yashtimadhu (Glycyrrhiza glabra) against side effects of radiation/chemotherapy in head and neck malignancies.
Cardioprotective effects of Glycyrrhiza uralensis extract against doxorubicin-induced toxicity.

ANTHELMINTIC
In vitro anthelmintic activity of an aqueous extract of Glycyrrhiza glabra and of glycyrrhetinic acid against gastrointestinal nematodes of small ruminants.

DENTAL CARIES / ORAL PATHOGENS
A Randomized, Double-Blind, Placebo-Controlled Clinical Trial of a Mouthwash Containing Glycyrrhiza uralensis Extract for Preventing Dental Caries.
Antimicrobial Effects against Oral Pathogens and Cytotoxicity of Glycyrrhiza uralensis Extract.
Preparation of Licorice Juice
This is conducted on a large scale in Spain, Southern France, Sicily, Calabria, Austria, Southern Russia (Astracan and Kasan), Greece (Patras) and Asia Minor (Sokia and Nazli, near Smyrna); but the extract with which England is supplied is almost exclusively the produce of Calabria, Sicily and Spain.

The process of manufacture varies only by reason of the amount of intelligence with which it is performed, and the greater or less perfection of the apparatus employed.  As witnessed by one of us at Rossano in Calabria in May, 1872, it may be thus described from notes made at the time. The factory employs about 60 persons, male and female. The root having been taken from the ground the previous winter, is stacked in the yard around the factory; it is mostly of the thickness of the fingers, with here and there a piece of larger size up to a diameter of nearly 2 inches; some of it sprouting.

As required, the root is taken within the building and crushed under a heavy millstone to a pulp, water-power being employed. It is then transferred to boilers and boiled with water over a naked fire. The decoction is run off and the residual root pressed in circular bags like those used in the olive-mills. The liquor which is received into cisterns below the floor is then pumped up into copper pans, in which the
evaporation is conducted also over the naked fire— even to the very last, care being taken by constant stirring to avoid burning the extract. The extract or pasta is removed from the pan while warm, and taken in small quantities to an adjoining apartment where a number of women are employed in rolling it into sticks. It is first weighed into portions, each of which the woman seated at the end of a long table tears with her hand into about a dozen pieces. These are passed to the women sitting next who roll them with their hands into cylindrical sticks, the table on which the rolling is done being of wood, and the pasta moistened with oil to prevent its adhesion to the hands. Near the further end of the table are some frames made of marble or metal, clean and bright, so arranged as to bring the sticks when rolled in them to the proper length and thickness. When thus adjusted, they are carefully ranged on a board, and a woman then stamps them with the name of the manufacturer. Lastly the sticks laid on boards are stacked up in a room to dry.

In some establishments the vacuum pan has been introduced for the inspissation of the decoction. At the great manufactory of Mr. A.O. Clarke at Sokia near Smyrna, all the processes are performed by steam power.’ (Pharmacographia, Fluckiger & Hanbury, 1879)